SH-U-454

An Efficient Method for Neuron-Like Differentiation of SH-SY5Y Neuroblastoma Cells Using Retinoic Acid and Laminin-Rich Extracellular Matrix.

Grigoli Marina Mantellatto MM, Pachane Bianca Cruz BC, Fuzer Angelina Maria AM, de Oliveira Sabrina Dorta SD et al.

The human neuroblastoma cell line SH-SY5Y is widely used in vitro as a model due to its ability to acquire neuron-like properties. Conventional differentiation protocols often rely on retinoic acid (RA), which can lead to limited stability of neuronal-like phenotypes over time. In this study, we evaluated a differentiation approach combining RA with a laminin-rich extracellular matrix (LrECM). This approach accelerated differentiation, with neuron-like morphology and neurite outgrowth observed as early as 4 days and sustained features for up to 10 days. Immunofluorescence analysis indicated increased NeuN expression, and Western blot confirmed the sustained presence of β3-tubulin during differentiation. Furthermore, a trend toward enhanced acetylcholinesterase (AChE) activity suggested partial cholinergic features. Altogether, these findings indicate that incorporating LrECM supports the stability of RA-induced neuron-like characteristics in SH-SY5Y cells and may provide a useful alternative for studies of neuronal-phenotype differentiation.

[The Clinical Significance and in vitro Experimental Study of hnRNP U in Multiple Myeloma].

Qi Wen-Hui WH, Xu Chun-Li CL, Zhang Li-Li LL, Tang Kou-Kou KK et al.

To analyze the expression level of heterogeneous nuclear ribonucleoprotein U (hnRNP U) and its correlation with prognosis in patients with multiple myeloma (MM), and explore the functional role as well as molecular mechanism of hnRNP U, thereby providing a theoretical basis for development of hnRNP U as a novel therapeutic target for MM. Based on Gene Expression Omnibus (GEO) database, the expression of hnRNP U in plasma cell diseases and healthy controls was compared. Based on the GSE9782 dataset (n =264), patients were divided into high expression group (n =114) and low expression group (n =150) according to the median expression level of hnRNP U . Overall survival (OS) between the two groups was compared. RPMI 8226, NCI-H929 and MM.1S cell lines were selected as tool cell lines. Following knockdown of hnRNP U by shRNA, the cell proliferation was detected by CCK-8. The apoptosis of MM cells was analyzed by Annexin V/7-AAD staining, and cell cycle was detected by BrdU/DAPI staining followed by flow cytometric analysis. The effect of hnRNP U on the biological characteristics of human MM cells was explored. The effect of knockdown of hnRNP U on DNA damage response pathways was analyzed by Western blot. Analysis of GSE5900 and GSE2113 datasets showed that the mRNA level of hnRNP U rose with the increased degree of malignancy of plasma cell diseases. Survival curve analysis of GSE9782 dataset showed that the high expression group of hnRNP U had a shorter OS than that of the low expression group. Down-regulation of hnRNP U in MM cell lines RPMI 8226, NCI-H929 and MM.1S could inhibit the cell proliferation, promote cell apoptosis and arrest the cell cycle. After knocking down hnRNP U, the expression levels of cleaved PARP and p-H2A.X, as the important markers of activated DNA damage pathway, were significantly increased in MM cells. hnRNP U is highly expressed in several plasma cell diseases including MM, and the high expression of hnRNP U in MM patients predicts poor prognosis. Knocking down hnRNP U can inhibit the malignant progression of MM cells, which is possibly associated with aggravated DNA damage.

Visual identification of Taiwan Mandarin rounded vowels.

Chiu Chenhao C

While auditory and visual cues jointly support vowel perception, the role of visual information alone in vowel identification remains understudied. This study examines how Taiwan Mandarin speakers identify rounded vowels using visual-only input. Results reveal an asymmetric pattern between /y/ and /u/, with a bias toward /u/ as the default rounded vowel and greater difficulty identifying /y/. Signal detection analyses further indicate a higher response criterion for /y/. This default preference for /u/ appears to reflect phonological distributions in the language or a universal preference for the more peripheral vowel. Varying degrees of visual input did not modulate identification strategies, and visual information provided only limited support for distinguishing between /u/ and /y/.

ASO Visual Abstract: Comparison Study of Inverted-Nipple Ureteroneocystostomy and Direct Anastomosis in U-Shaped Orthotopic Neobladder Reconstruction.

Xing Qi Q, Wei Yang-Bing YB, Wang Xin X, Hao Hai-Feng HF et al.