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sildenafil (Liqrev)

✓ Approved

CMP Pharma, Inc. · PDE5A · 小分子

什么是 sildenafil？

sildenafil 是一种小分子，由CMP Pharma, Inc.研发。该药已获批，用于治疗相关适应症，给药途径：Oral (PO)。

药物档案

商品名Liqrev
公司CMP Pharma, Inc.
药物类别小分子
分子靶点PDE5A
给药途径Oral (PO)
状态Approved
来源： ClinicalTrials.gov, CMP Pharma, Inc.

作用机制

分子靶点

sildenafil 作用于 1 个分子靶点：

PDE5Aphosphodiesterase 5A (PDE5, CGB-PDE)
需要更深入的分析？Noah AI 可解释复杂机制并与同类药物比较。

治疗适应症

sildenafil 针对 1 个适应症，涉及 1 个治疗领域。

治疗领域疾病/病症分期
Respiratory, thoracic and mediastinal disordersPulmonary hypertension✓ Approved

相关研究文献

PubMedMethods (San Diego, Calif.)2026-08-04

Europium(III)-driven red photoluminescent sensor for ultra-sensitive and reliable assay of sildenafil citrate in pure form, pharmaceutical formulations and Bio-fluids.

Ali Hazim M HM, Essawy Abd El-Naby I AEI, Elnasr Tarek Ahmed Seaf TAS, Essawy Amr A AA

Lanthanide complexes, particularly Eu3+ β-diketonates, offer narrow-band, long-lived red emission ideally suited to sensitive fluorescence assays in complex media. The Eu(TTA)3(H2O)2 chelate combines efficient near-UV excitation, robust emission and labile coordination sites, making its signal highly responsive to molecular binding events. Sildenafil citrate is a widely used PDE-5 inhibitor whose misuse, counterfeiting and potential drug interactions demand reliable monitoring in pharmaceuticals and biological fluids. However, current chromatographic techniques are costly and labor-intensive, creating a pressing need for a simple, robust, low-cost, green, field-deployable optical method for sildenafil quantification. Near-UV excitation around 350 nm efficiently promotes ligand-to-metal energy transfer to the Eu3+ 5D0 level, producing line-narrowed 5D0→7F2 emission at ∼ 614 nm whose intensity is selectively quenched by sildenafil without disturbing its spectral fingerprint. The interaction between the Eu-TTA chemosensor and sildenafil involves a mixed mechanism of dynamic fluorescence deactivation and static "antenna" shelving, governed by photo-induced electron transfer and triplet-triplet energy transfer. Under optimized experimental conditions, the sensing protocol exhibits excellent linear Stern-Volmer behavior for sildenafil concentrations from 0.2 to 8.0 µg mL-1 (r ≈ 0.999), with a limit of detection of 0.036 µg mL-1 and a limit of quantification of 0.121 µg mL-1. Fluorimetric method coupled with SupelcleanTM LC-Florisil SPE cartridge with 2-propanol elution solvent was used for the selective determination of sildenafil in human serum and urine avoiding the presence of interference. Analysis of sildenafil in pharmaceutical formulations, spiked human urine and serum affords high overall accuracy and precision, with recoveries of 95.26-99.12 %, 97.5-102.4 % and 93.7-102.3 % and RSD values of 1.29-1.35 %, 1.16-2.01 and 1.21-2.71, respectively, while AGREE and BAGI metrics confirm the method's strong greenness and practical applicability. Consequently, the Eu-TTA-based fluorimetric probe provides a simple, sensitive, and low-cost analytical platform for sildenafil determination in pharmaceutical formulations and biological matrices, with satisfactory accuracy, precision, and practical applicability. Moreover, the system probing performance surpasses existing spectrophotometric methods and rivals chromatographic techniques in sensitivity while offering significant operational simplicity, high throughput, and cost efficiency, thus providing a green and clinically relevant alternative platform.

PMID 42546935
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PubMedOxford medical case reports2026-08-04

Protein-losing enteropathy in a child with hypoplastic left heart syndrome after Fontan palliation: a case report.

Saife Sakeena S, Saife Sarah S, Zetawi Momen M

Protein-losing enteropathy (PLE) is a rare but serious complication of the Fontan procedure in patients with single-ventricle physiology. It results from excessive loss of plasma proteins into the gastrointestinal tract, leading to hypoalbuminemia, edema, and ascites. We report the case of a 10-year-old girl with hypoplastic left heart syndrome who underwent staged surgical palliation including the Norwood procedure, bidirectional Glenn shunt, and extracardiac Fontan completion. She presented with progressive abdominal distension, peripheral edema, and severe hypoalbuminemia (albumin 2.3 g/dl). Echocardiography and cardiac catheterization demonstrated a patent Fontan circuit with normal pulmonary artery pressures and no significant obstruction, although the inferior vena cava pressure was mildly elevated at approximately 16 mmHg. The patient was managed with intravenous albumin replacement, diuretics, anticoagulation, sildenafil, and oral budesonide. This case highlights the diagnostic challenges of PLE and emphasizes the importance of early recognition and multidisciplinary management, even in the absence of significant Fontan circuit obstruction, as elevated systemic venous pressure and lymphatic dysfunction may both contribute to disease development. Protein-losing enteropathy is a rare but potentially life-threatening complication of Fontan circulation. It should be suspected in patients with a history of Fontan palliation who present with unexplained hypoalbuminemia, edema, or ascites, even when no significant Fontan circuit obstruction is identified, as elevated systemic venous pressure and lymphatic abnormalities may contribute to disease development. Early recognition and multidisciplinary management are essential to improve clinical outcomes and guide further evaluation of underlying lymphatic abnormalities.

PMID 42549227
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PubMedDoklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections2026-08-04

Optimization of Exosome Isolation from Vitis vinifera Cell Cultures.

Sorokina M R MR, Fialko A V AV, Yugay Yu A YA, Grishchenko O V OV et al.

Plant extracellular vesicles (exosomes) are considered promising nanostructures for biomedical and biotechnological applications; however, methods for their isolation from plant cell cultures remain insufficiently optimized. The aim of this study was to evaluate the efficiency of exosome isolation from callus and suspension cultures of Vitis vinifera and to investigate the effects of culture age and extraction method on the yield of extracellular vesicles. It was demonstrated that suspension culture provides a higher exosome yield compared to callus tissue. Isolated particles were characterized by a size of 90-120 nm and a negative zeta potential, which corresponded to typical properties of plant extracellular vesicles. The maximum exosome yield was observed with a two-week-old suspension culture, which corresponded to the phase of intense cell growth. Comparison of extraction methods revealed that mechanical homogenization using a blender was the most efficient approach for exosome isolation, providing a high particle yield without significant deterioration in sample purity. The results demonstrate the potential of V. vinifera suspension cultures as a scalable platform for the production of plant-derived exosomes and may be useful for the development of biotechnological systems for the isolation of plant extracellular vesicles.

PMID 42550421
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PubMedCell reports. Medicine2026-08-04

EGFR inhibition promotes functional engraftment and graft survival via donor-derived CHI3L1 in fetal RPE suspension transplantation.

Mao Xiying X, Yin Wenjie W, Xu Miao M, Niu Zixuan Z et al.

Numerous clinical trials have highlighted the translational potential of retinal pigment epithelial (RPE) cell transplantation in retinal degeneration (RD). However, an unsolved challenge remains in addressing chronic graft dysfunction, primarily due to poor integration and cell loss. Here, we perform a single-cell transcriptomic comparison between grafted RPE cells and host RPE. Our analysis identifies EGFR expression in donor fetal RPE cells as a significant obstacle to functional integration. Through multimodal imaging and spatial transcriptomics, we demonstrate that EGFR inhibition (EGFRi) facilitates effective engraftment of fetal RPE cell suspension, leading to the functional restoration of the outer blood-retinal barrier, and restrains neurodegeneration in preclinical RD models. This effect is mechanistically linked to graft reepithelialization via the activation of intrinsic CHI3L1-IL13RA2 signaling in EGFRi-treated donor cells. Moreover, EGFRi-induced CHI3L1 secretion contributes to immunosuppression that complements anti-lymphocytic therapy, thereby extending graft survival. Thus, repurposing Food and Drug Administration (FDA)-approved EGFR inhibitor offers a safe and cost-effective approach to advancing fetal RPE suspension transplantation into clinical practice.

PMID 42546699
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PubMedBiofabrication2026-08-04

Development of a low cell density model for examining astrocyte reaction to 3D suspension using AVIFJ.

Smith Austin A, Song Yu Y, Sun Wei W, Lin Feng F

Alternating Viscous and Inertial Force Jetting (AVIFJ) was used to print single-cells and position small numbers of cells precisely. The effect of nozzle size on single-cell encapsulation and droplet spacing ability was tested using astrocytes. Astrocytes were patterned in Matrigel and examined for 7 Days after printing to assess viability and their response to being suspended in 3D at low cell density. The results showed AVIFJ had a single-cell printing efficiency around 30% using nozzles with outer diameters (OD) of 63 μm and 96 μm at a 300,000 cells/ml concentration, and 30% for 143 µm and 195 µm OD nozzles at a 50,000 cells/ml concentration. Spacing using a 63 μm nozzle tests printed droplets with a minimum center-to-center spacing of 199.53±2.52 μm on a culture well and cell spacing of 122.23±21.69 μm in 3D. Astrocyte viability was 93.60±.27% when printed into Matrigel. RNA sequence results showed astrocytes suspended Matrigel upregulated IGFBP3 compared to 2D controls. Results also showed that astrocytes suspended in 3D that contact the culture well react differently than those completely suspended over 4 Days. Overall, this study showed the viability of AVIFJ printing for low cell number experiments and differences in astrocyte behavior in 3D suspension compared to 2D.

PMID 42546761
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PubMedCommunity dentistry and oral epidemiology2026-08-04

Can a Low-Threshold Check-Up Motivate Older Adults to Schedule a Dental Visit? A Randomized Controlled Trial.

De Vleeschauwer Aster A, Colman Roos R, Pype Peter P, Poppe Louise L et al.

Oral disorders are an important disability driver in adults aged 70 and older, who often experience greater tooth loss and higher levels of untreated oral disease than younger persons. Despite the importance of regular dental visits, older adults attend dental services less frequently, facing several barriers. This study aimed to evaluate whether a low-threshold dental screening could increase the likelihood that older adults (≥ 65 years) contact a dental professional. A total of 194 community-dwelling adults aged ≥ 65 years without a dental check-up in the past 12 months were randomized (1:1) in a two-arm, single-blinded, controlled superiority trial. The intervention group received an oral screening, personalized oral health information, referral letters for a dentist and general practitioner, and a list of nearby dentists. The control group received only general oral hygiene flyers and a list of nearby dentists. The primary outcome was whether participants contacted a dental professional within 4 months following the intervention. Oral hygiene practices (i.e., tooth brushing frequency and use of brushing materials) were evaluated as secondary outcomes. No evidence for an effect of the intervention on contact with a dental professional was found (risk difference = -0.01 (95% CI: -0.12; 0.10)). Within 4 months, 14% of control group participants and 12% of those in the intervention group reported contacting a dental professional, mostly due to urgent issues (e.g., pain or broken teeth), rather than the intervention itself. Among those who did not seek care (n = 133), 79% cited a lack of perceived need. Furthermore, no intervention effect was found regarding oral hygiene practices. Motivating older adults to attend preventive professional dental care remains challenging. Greater integration of oral health into primary care, along with early-life promotion of positive oral health behaviours, is essential.

PMID 42548003
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