若生智能
Drug Database
CO

collagenase (Plaquase / Nucleolysin / lipolysin)

✓ Approved

Johnson & Johnson Services, Inc. · 治疗药物

什么是 collagenase？

collagenase 是一种治疗药物，由Johnson & Johnson Services, Inc.研发。该药已获批，用于治疗相关适应症，给药途径：Injectable (Others)、Topical。

药物档案

商品名Plaquase, Nucleolysin, lipolysin
公司Johnson & Johnson Services, Inc.
给药途径Injectable (Others), Topical
状态Approved
来源： ClinicalTrials.gov, Johnson & Johnson Services, Inc.

治疗适应症

collagenase 针对 10 个适应症，涉及 7 个治疗领域。

治疗领域疾病/病症分期
Skin and subcutaneous tissue disordersDecubitus ulcer✓ Approved
Injury, poisoning and procedural complicationsThermal burn✓ Approved
Skin and subcutaneous tissue disordersDiabetic foot✓ Approved
Musculoskeletal and connective tissue disordersDupuytren's contracture✓ Approved
Eye disordersGlaucoma✓ Approved

注册免费账户还可查看另外 5 个适应症

免费注册查看全部适应症 →

相关研究文献

PubMedBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences2026-06-13

[A new method for extracting adult mouse cardiac fibroblasts more efficiently and stably].

Ma Xiaojuan X, Wang Hao H, Ma Xueqin X, Song Ying Y et al.

Cardiac fibroblasts (CFs) play a central role in myocardial remodeling and fibrosis. Efficient isolation of CFs is a prerequisite for investigating related mechanisms. However, current methods for isolating primary adult mouse CFs suffer from prolonged processing time, low yield, and poor viability. This study aims to establish a rapid, high-yield, and stable isolation protocol for adult mouse CFs by optimizing the synergistic effect of enzymatic digestion and mechanical dissociation parameters. Using the gentleMACS® Octo Dissociator with Heaters, we selected different types and concentrations of collagenase, trypsin, and nuclease as the enzymatic digestion system for CFs extraction. We explored the optimal extraction conditions and compared the results with the commercial Multi Tissue Dissociation Kit 2. The cell yield was quantified using a high-content imaging analysis system by counting the number of adherent cells per field after 72 h of culture. The CFs purity was assessed using immunofluorescence staining for vimentin. The trans-differentiation activity of the CFs was evaluated with transforming growth factor β1 (TGF-β1). Omitting any component of the digestion solution (collagenase Ⅱ/Ⅳ, trypsin or DNaseⅠ), significantly prolonged extraction time and reduced cell yield. In contrast, the optimized protocol outperformed the commercial kit, reducing digestion time by 32.2 min and significantly increasing cell yield, and with comparable obtained CFs purity. After TGF-β1 stimulation, CFs exhibited enhanced proliferative capacity and upregulated expression of α-smooth muscle actin (α-SMA), collagen type Ⅰ (ColⅠ), and fibronectin (FN), confirming the differentiation potential of CFs isolated via the optimized method. This study systematically optimized an enzymatic digestion method combining collagenase, trypsin, and nuclease in conjunction with mechanical dissociation using a tissue dissociator, leading to the efficient and stable isolation of adult mouse CFs. By fine-tuning enzyme concentrations and digestion conditions, we successfully reduced processing time, improved cell yield, and enhanced cell viability compared with conventional isolation methods. These findings validate the physiological relevance of the isolated CFs and demonstrate that the optimized protocol provides a reliable and reproducible method for studying myocardial fibrosis and remodeling. This protocol can serve as a valuable tool for researchers investigating CFs biology and its role in cardiovascular diseases.

PMID 42287063
阅读全文 →
PubMedBiomaterials advances2026-06-13

Supramolecular guest-host composites of adamantane-modified gelatin and β-cyclodextrin polymers, displaying unexpected rheological and bioinert behavior.

Raeymaekers Ruben R, Valoor Remya R, Vergauwen Bjorn B, Prakash Jai J et al.

Because of their strong complexation behavior, guest-host interactions between adamantane (Ad) and β-cyclodextrin (CD) are often used to create physically crosslinked, reversible supramolecular networks. In this work, scalable syntheses of adamantane-modified gelatin (GelAd) and high molecular weight, water-soluble β-cyclodextrin polymers (CDPs) are presented. Despite confirmed Ad-CD complexation, guest-host combinations of GelAds and CDPs resulted in viscous liquids with viscosities up to tens of Pa.s in all cases at physiological temperature. The resulting viscous composites showed strong shear-thinning and excellent self-healing behavior, but consistently higher loss moduli than storage moduli (G″ > G'), even at high polymer concentrations, high degrees of GelAd modification, and with CDPs near the solubility-limited molecular weight. The addition of laponite enabled true solid hydrogel formation at physiological temperature, but the resulting solid hydrogel was not completely self-healing. The GelAd-CDPs composites exhibited improved resistance to degradation by collagenase. Surprisingly, the composites exhibited no cellular attachment by NIH/3T3 fibroblasts despite the presence of native RGD motifs on gelatin. In addition, the NIH/3T3 fibroblasts remained viable near the gel interface over 12 days, indicating no cytotoxicity and making them highly bioinert. These results reveal that Ad-CD guest-host interactions were in this case not sufficient to form truly solid hydrogels at physiological temperature. Instead, strongly viscous soft composites were formed. The unique combination of shear-thinning, self-healing, improved collagenase resistance, cytocompatibility and bioinertness highlights these GelAd-CDP composites as promising materials for biomedical applications requiring cell-repellent and mechanically adaptive interfaces.

PMID 42284757
阅读全文 →
PubMedFoods (Basel, Switzerland)2026-06-12

Bioresource Potential and Safety Evaluations of Thai Zea mays L. Husk Waste Extracts.

Sainakham Mathukorn M, Chaiyana Wantida W, Kiattisin Kanokwan K, Somwongin Suvimol S et al.

Corn husk, a prevalent agricultural byproduct, remains an underutilized source of bioactive compounds. This study investigated the influence of extraction solvents (water, 50% ethanol, and 95% ethanol) and techniques (maceration, reflux, ultrasound-assisted extraction) on the phytochemical profiles and biological activities of corn husk. The results revealed that water extraction produced the highest total phenolic content, whereas 95% ethanol extraction yielded the greatest total flavonoid content and the most potent antioxidant activity in DPPH testing. In contrast, ultrasound-assisted water extraction exhibited the most potent nitric oxide inhibition (94.62 ± 2.13%) and tyrosinase inhibition (IC50 = 7.54 ± 0.27 mg/mL), indicating anti-inflammatory and skin-lightening potential. This extract showed anti-collagenase activity (91.49 ± 4.01%), outperforming ascorbic acid (29.79 ± 1.00%) and EGCG (82.27 ± 1.00%), though its anti-hyaluronidase activity was limited. Cytotoxicity testing revealed cytotoxicity at 10 mg/mL, while the HET-CAM assay confirmed non-irritation at the same level. These findings underscore that ultrasound-assisted water extraction is a safe and successful technique for obtaining bioactive-rich extracts. This study supports the transition of corn husk from agricultural waste to a high-value, safe, and multifunctional natural ingredient for the cosmetic, pharmaceutical, and functional food industries.

PMID 42279695
阅读全文 →
PubMedPain and therapy2026-06-12

Research Progress on Low-Temperature Plasma Nucleoplasty and Targeted Radiofrequency for Cervical Spondylotic Radiculopathy: A Retrospective Review.

Deng Jiajia J, Xiao Chenxuan C, Xie Keyue K

Cervical spondylotic radiculopathy (CSR) is a common neurological condition caused by compression or inflammation of cervical nerve roots, often resulting in significant pain and functional impairment. While many patients improve with conservative management, a substantial subset remains refractory and requires further intervention. Over the past 2 decades, minimally invasive percutaneous techniques have emerged as effective alternatives to conventional open surgery. This review synthesizes contemporary evidence on two key modalities: percutaneous cervical nucleoplasty (PCN), a decompressive procedure utilizing low-temperature plasma (coblation) technology to reduce intradiscal pressure, and pulsed radiofrequency (PRF) applied to the dorsal root ganglion, a neuromodulatory approach that modulates pain signaling without tissue destruction. Drawing on observational studies, randomized controlled trials, and systematic reviews, the article evaluates the clinical efficacy, safety, and durability of each technique, compares their outcomes with anterior cervical discectomy and fusion, and explores emerging combination strategies. Evidence indicates that both PCN and PRF provide significant and sustained pain relief with favorable safety profiles. PCN achieves long-term outcomes comparable to surgery in carefully selected patients with contained disc herniations, while PRF offers superior duration of relief compared with steroid injections alone. Emerging data support the synergistic use of PCN and PRF, as well as augmentation with intradiscal collagenase or ozone. Patient selection-guided by symptom duration, imaging characteristics, and predictive factors-remains critical for optimizing outcomes. Despite encouraging results, the evidence base is limited by study heterogeneity and moderate-to-low certainty. Future large-scale, multicenter randomized trials are needed to refine patient selection, optimize technical parameters, and establish comparative effectiveness. Overall, PCN and PRF represent valuable, minimally invasive options within a stepped-care approach to CSR, bridging conservative management and open surgery.

PMID 42277358
阅读全文 →
PubMedBiochemical and biophysical research communications2026-06-11

Activated B lymphocytes induce hepatocyte injury in autologous co-culture system.

Guan Weiwei W, Lv Zhuman Z, Peng Hui H, Gao Han H et al.

Hepatic B cells are increasingly implicated in liver inflammation and fibrosis, but whether activated hepatic B cells aggravate primary hepatocyte injury remains insufficiently defined. We established a paired same-donor co-culture system using primary hepatocytes and hepatic B cells isolated from the same mouse liver. Primary hepatocytes were obtained from perfused liver lobes by blood clearance, collagenase I digestion, filtration, and Percoll gradient purification. Hepatic B cells were isolated from paired liver lobes after rapid cold phosphate-buffered saline (PBS)-based washing to remove residual blood, tissue mincing, collagenase IV digestion, red blood cell lysis, Percoll gradient purification, and negative magnetic B-cell enrichment. The workflow yielded viable primary hepatocytes and hepatic B cells with CD19-positive purity above 90% after magnetic sorting and 72-h culture. Under optimized co-culture conditions, lipopolysaccharide (LPS)-activated hepatic B cells secreted TNF-α, IL-6, and IL-1β in culture supernatants and induced hepatocyte morphological damage, increased terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positivity, and upregulation of cleaved caspase-3. These findings support activated hepatic B cells as inflammatory amplifier cells that can promote hepatocyte injury and apoptosis through soluble inflammatory mediators in a same-donor primary-cell system.

PMID 42269273
阅读全文 →
PubMedThe Journal of hand surgery2026-06-11

The Likelihood of Future Dupuytren Disease Intervention After Initial Treatment in the Same Digit, Another Digit, and Contralateral Hand.

El Bachaoui Rayan R, Cossu Ethan E, Zhang Dafang D, Earp Brandon E BE et al.

Patients treated for Dupuytren contracture in one digit are at risk for future intervention in other digits or the contralateral hand. Identifying predictive factors can aid in patient counseling. A single institutional database was queried for procedural codes for collagenase treatment or surgical fasciectomy. A total of 658 patients were identified in this query. Out of this cohort, 341 patients were eligible for inclusion. The cohort consists of 141 initially treated with fasciectomy and 200 initially treated with collagenase, between 2015 and 2019. The primary outcome was the need for subsequent treatment in the same digit, other digits of the same hand, or the contralateral hand. Predictor variables included demographics, medical history, and pretreatment contracture severity. At a mean follow-up of 5.3 years, 142 (42%) of patients underwent additional treatment. Revision procedures on the same digit occurred in 88 patients (26%) at a mean of 3.2 years, on another digit in the same hand in 35 patients (10%) at a mean of 3.6 years, and on the contralateral hand in 79 patients (23%) at a mean of 2.3 years. Procedure, multiple digits treated, and initial nontreated contracture are associated with subsequent procedures on the same digit. Younger age, active smoking, and initial nontreated contracture are associated with subsequent procedures on another digit in the same hand. Dominant hand was initially treated; initial nontreated contracture are associated with subsequent procedures on the contralateral hand. Within 5 years of initial treatment for Dupuytren contracture in one digit, patients have over 40% likelihood of future intervention for contracture. Patients have approximately a 10% likelihood of future treatment on another digit in the same hand and nearly a 25% likelihood of future treatment in the contralateral hand. Key risk factors include age, multiple digits treated, smoking, and the presence of initially nontreated fingers, which should be incorporated into patient counseling. 3-Retrospective cohort study, prognostic.

PMID 42274446
阅读全文 →

注册免费账户还可查看另外 9996 篇文献

免费注册查看全部文献 →

了解更多collagenase